Amniotic stem cells demonstrate healing potential

April 9, 2015 by Mike Williams, Rice University
The lab of Rice University and Texas Children’s Hospital bioengineer Jeffrey Jacot has successfully used stem cells derived from amniotic fluid to promote the growth of functional blood vessels in an injectable hydrogel. Credit: Jeff Fitlow/Rice University

Rice University and Texas Children's Hospital scientists are using stem cells from amniotic fluid to promote the growth of robust, functional blood vessels in healing hydrogels.

In new experiments, the lab of bioengineer Jeffrey Jacot combined versatile amniotic with injectable hydrogels used as scaffolds in regenerative medicine and proved they enhance the development of vessels needed to bring blood to new tissue and carry waste products away.

The results appear in the Journal of Biomedical Materials Research Part A.

Jacot and his colleagues study the use of from pregnant women to help heal infants born with . Such fluids, drawn during standard tests, are generally discarded but show promise for implants made from a baby's own genetically matched material.

He contends amniotic stem cells are valuable for their ability to differentiate into many other types of cells, including that form blood vessels.

"The main thing we've figured out is how to get a vascularized device: laboratory-grown tissue that is made entirely from amniotic fluid cells," Jacot said. "We showed it's possible to use only cells derived from amniotic fluid."

In the lab, researchers from Rice, Texas Children's Hospital and Baylor College of Medicine combined stem cells with a hydrogel made from and fibrin. Fibrin is a biopolymer critical to blood clotting, cellular-matrix interactions, wound healing and angiogenesis, the process by which new vessels branch off from existing ones. Fibrin is widely used as a bioscaffold but suffers from low mechanical stiffness and rapid degradation. Combining fibrin and polyethylene glycol made the hydrogel much more robust, Jacot said.

A microscope image shows mature blood vessels that formed in a hydrogel after two weeks of growth in a mouse model, with red blood cells flowing through the vessel at bottom right. The vessels form from stem cells derived from amniotic fluid in a technique created by the lab of Rice University and Texas Children’s Hospital bioengineer Jeffrey Jacot. The scale bar is 100 microns. Credit: Jacot Lab/Rice University

The lab used vascular endothelial growth factor to prompt stem cells to turn into endothelial cells, while the presence of fibrin encouraged the infiltration of native vasculature from neighboring tissue.

Mice injected with fibrin-only hydrogels showed the development of thin fibril structures, while those infused with the amniotic cell/fibrin hydrogel showed far more robust vasculature, according to the researchers.

A hydrogel seeded with amniotic fluid stem cells shows the growth of mature blood vessels two weeks after implantation in an experiment by researchers at Rice University and Texas Children’s Hospital. The cell nuclei are blue, endothelial cells are red and smooth muscle cells are green. The scale bar is 20 microns. Credit: Jacot Lab/Rice University

Similar experiments using hydrogel seeded with bone marrow-derived mesenchymal cells also showed vascular growth, but without the guarantee of a tissue match, Jacot said. Seeding with endothelial cells didn't work as well as the researchers expected, he said.

The lab will continue to study the use of to build biocompatible patches for the hearts of infants born with birth defects and for other procedures, Jacot said.

More information: "In situ vascularization of injectable fibrin/poly(ethylene glycol) hydrogels by human amniotic fluid-derived stem cells." Journal of Biomedical Materials Research Part A DOI: 10.1002/jbm.a.35402

Provided by Rice University