Stem cell study offers clues for optimizing bone marrow transplants and more

After radiation, a small number of blood stem cells make an outsized contribution to reconstituting the blood and immune system. Credit: Figure by Jiya Eerdeng/Rong Lu Lab/USC Stem Cell

Bone marrow transplants, which involve transplanting healthy blood stem cells, offer the best treatment for many types of cancers, blood disorders and immune diseases. Even though 22,000 of these procedures are performed each year in the US, much remains to be understood about how they work.

A new USC and Stanford study, conducted in mice, deepens the mystery, showing that successfully transplanted don't behave "normally" as in a healthy person without a transplant. Instead, the radiation and high-dose chemotherapy used to wipe out diseased stem cells prior to transplantation appear to trigger "extreme behavior" in the newly transplanted cells.

The findings appeared in Proceedings of the National Academy of Sciences (PNAS) on January 8.

"Our research has important implications for understanding and optimizing and certain types of gene therapy," said lead researcher and co-corresponding author Rong Lu, assistant professor of stem cell biology and regenerative medicine at USC. The other co-corresponding author is Irving Weissman, director of the Stanford Institute for Stem Cell Biology and Regenerative Medicine.

In a series of experiments, scientists learned that when transplanted into an irradiated mouse, only a very small minority of the stem cells produce blood and immune cells, while many other stem cells become dormant and do nothing. In addition, post-radiation, this handful of "super producer" stem cells also become biased towards producing only certain types of . However, the overall blood and , still tends to remain balanced.

In mice that had not undergone radiation, all stem cells contributed equally to the blood and immune systems, with the exception of T cells, suggesting that the preconditioning regimen used to ensure successful transplantation is the source of the abnormal cell behavior.

More information: Rong Lu et al, Clonal-level lineage commitment pathways of hematopoietic stem cells in vivo, Proceedings of the National Academy of Sciences (2019). DOI: 10.1073/pnas.1801480116

Journal information: Proceedings of the National Academy of Sciences

Provided by University of Southern California